Composite

Part:BBa_J23073:Design

Designed by: John Anderson   Group: iGEM06_Berkeley   (2006-08-20)

lock3k RFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 677
    Illegal AgeI site found at 789
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

n/a


Source

PCR ca1028F/ca1001 on pJ23019-J23072 (865 bp, XbaI/PstI)
Sub into pJ23076 (SpeI/PstI)
Product is pJ23019-J23073, [Ptet][lock3k][RFP]

ca1028F Forward XbaI to extend lock3 variants
gcatgTCTAGAGtgtagaccgAACTAGAATCACCTCTTGC

References